Acid Cardiolipin and an Improved Method for the Preparation of Cardiolipin from Beef Heart
نویسنده
چکیده
In a previous report (1) the isolation of a new phospholipid from beef heart was described. The name “cardiolipin” was suggested for this substance, which is of particular interest in connection with serodiagnostic tests for syphilis (2). The original method of preparation was tedious and involved considerable losses. An improved and simplified method is here described. The development of the new method depended in part on a study of the properties of the acid form of cardiolipin, the preparation of which is now reported for the first time. The results of a study of the cleavage products of cardiolipin will be reported later. It should be noted at this time, however, that the original report of the presence of a carbohydrate component in the molecule was in error. The results obtained were apparently due to persistent traces of carbohydrate impurities.
منابع مشابه
Novel preparation of cardiolipin from beef heart.
A new method is described for the isolation of beef heart cardiolipin. A lipid-protein complex, rich in cardiolipin, is obtained by a one-step solvent fractionation of the tissue total lipid extract. Cardiolipin in the complex is largely freed of protein by salt denaturation and is further purified by gel filtration on Sephadex LH-20 followed by column chromatography on bicarbonate-treated sili...
متن کاملImmunological study on cardiolipin from Escherichia coli.
For the first time we found that cardiolipin was contained abundantly in Eschrichia coli, and we succeeded in isolating and purifying it as reported previously. With this E. coli cardiolipin a study was made on its reactivity to Wassermann antibody reagin by OGATA’S box titration, and the following results were obtained. L The purity of cardiolipin prepared from E. coli has been found to be sat...
متن کاملUse of an Antibody to Study the Location of Cardiolipin in Mitochoudrial Membranes
Rabbit antiserum to cardiolipin, which is reactive with the polar head but not the nonpolar fatty acid moieties of cardiolipin, was used to explore the location of the polar head of cardiolipin in mitochondrial membranes. Only a few per cent of the cardiolipin in intact mitochondria from rat liver, blowfly flight muscle, Saccharomyces cerevisiae, and Neurospora and none of the cardiolipin in in...
متن کاملUse of an antibody to study the location of cardiolipin in mitochondrial membranes.
Rabbit antiserum to cardiolipin, which is reactive with the polar head but not the nonpolar fatty acid moieties of cardiolipin, was used to explore the location of the polar head of cardiolipin in mitochondrial membranes. Only a few per cent of the cardiolipin in intact mitochondria from rat liver, blowfly flight muscle, Saccharomyces cerevisiae, and Neurospora and none of the cardiolipin in in...
متن کاملThe cardiolipin-lecithin test for syphilis.
as the antigen for a simplified precipitation test for syphilis. Cardiolipin is the lipid which, in combination with lecithin, gives the alcoholic extract of beef heart its antigenic properties. It is a complex phosphatidic acid with a molecular weight of 2195 and iodine number 120-125. It requires lecithin and cholesterol for antigenicity. It has not yet been synthesized. The cardiolipin-lecit...
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